KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
5-[(1E)-3-[(4-[[(5-azido-2-nitrophenyl)carbonyl]amino]butanoyl)amino]prop-1-en-1-yl]uridine 5'-triphosphate | pH 9.0, 25°C | Thermus thermophilus | |
0.002 | - |
dTTP | pH 9.0, 25°C | Thermus thermophilus | |
0.0055 | - |
5-[(1E)-3-{[(5-azido-2-nitrophenyl)carbonyl]amino}prop-1-en-1-yl]-2'-deoxyuridine 5'-triphosphate | pH 9.0, 25°C | Thermus thermophilus | |
0.04 | - |
5-[N-(2-nitro-5-azidobenzoyl)ami-nomethyl]-2'-deoxyuridine 5'-triphosphate | pH 9.0, 25°C | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus B35 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-[(1E)-3-[(4-[[(5-azido-2-nitrophenyl)carbonyl]amino]butanoyl)amino]prop-1-en-1-yl]uridine 5'-triphosphate + DNAn | - |
Thermus thermophilus | ? | - |
? | |
5-[(1E)-3-[(4-[[(5-azido-2-nitrophenyl)carbonyl]amino]butanoyl)amino]prop-1-en-1-yl]uridine 5'-triphosphate + DNAn | - |
Thermus thermophilus B35 | ? | - |
? | |
5-[(1E)-3-{[(5-azido-2-nitrophenyl)carbonyl]amino}prop-1-en-1-yl]-2'-deoxyuridine 5'-triphosphate + DNAn | - |
Thermus thermophilus | ? | - |
? | |
5-[(1E)-3-{[(5-azido-2-nitrophenyl)carbonyl]amino}prop-1-en-1-yl]-2'-deoxyuridine 5'-triphosphate + DNAn | - |
Thermus thermophilus B35 | ? | - |
? | |
5-[N-(2-nitro-5-azidobenzoyl)ami-nomethyl]-2'-deoxyuridine 5'-triphosphate + DNAn | - |
Thermus thermophilus | ? | - |
? | |
5-[N-(2-nitro-5-azidobenzoyl)ami-nomethyl]-2'-deoxyuridine 5'-triphosphate + DNAn | - |
Thermus thermophilus B35 | ? | - |
? | |
deoxynucleoside triphosphate + DNAn | several dTTP analoges bearing a photoreactive 2-nitro-5-azidobenzoyl group attached at position 5 of uracil through linkers of various lengths, are substrates in the elongation reaction of the 5'-32P-labeled primer-template complex. The incorporation of the analogs into the 3' primer end did not impede further elongation of the chain in the presence of natural dNTP | Thermus thermophilus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | several dTTP analoges bearing a photoreactive 2-nitro-5-azidobenzoyl group attached at position 5 of uracil through linkers of various lengths, are substrates in the elongation reaction of the 5'-32P-labeled primer-template complex. The incorporation of the analogs into the 3' primer end did not impede further elongation of the chain in the presence of natural dNTP | Thermus thermophilus B35 | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | - |
Thermus thermophilus | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | - |
Thermus thermophilus B35 | diphosphate + DNAn+1 | - |
? |