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Literature summary for 2.7.7.7 extracted from

  • Tuske, S.; Singh, K.; Kaushik, N.; Modak, M.J.
    The J-helix of Escherichia coli DNA polymerase I (Klenow fragment) regulates polymerase and 3'-5'-exonuclease functions (2000), J. Biol. Chem., 275, 23759-23768.
    View publication on PubMed
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
N678A no change in polymerase activity, increased mismatch-directed exonuclease activity Escherichia coli
P680G reduced kcat, no change in relative DNA binding affinity or Km, nearly complete loss in the processive mode of DNA synthesis Escherichia coli
P680Q reduced kcat, no change in relative DNA binding affinity or Km, nearly complete loss in the processive mode of DNA synthesis Escherichia coli
Q667A polymerase defective, no change in exonuclease activity Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Escherichia coli
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P00582
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Escherichia coli diphosphate + DNAn+1
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli