Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Thermus aquaticus |
wild-type and mutant enzymes | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
G418K | increased exonuclease activity | Thermus aquaticus |
Q507E | increased exonuclease activity | Thermus aquaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimal concentration: 50 mM | Thermus thermophilus | |
K+ | optimal concentration for wild-type enzyme: 50 mM, optimal concentration for G418/E507Q mutant: 100 mM | Thermus aquaticus | |
Mg2+ | optimal concentration: 8 mM | Thermus aquaticus | |
Mg2+ | optimal concentration: 8 mM | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Thermus aquaticus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Thermus thermophilus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Thermus aquaticus |
wild-type and mutant enzymes | Thermus thermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | binding mechanism | Thermus aquaticus | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | binding mechanism | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermus aquaticus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Thermus thermophilus | diphosphate + DNAn+1 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0667 | - |
deoxynucleoside triphosphate | wild-type enzyme, pH 7.5, 60°C | Thermus aquaticus | |
0.0667 | - |
deoxynucleoside triphosphate | wild-type enzyme, pH 7.5, 60°C | Thermus thermophilus | |
0.15 | - |
deoxynucleoside triphosphate | G418K/E507Q mutant, pH 7.5, 60°C | Thermus aquaticus | |
0.15 | - |
deoxynucleoside triphosphate | G418K/E507Q mutant, pH 7.5, 60°C | Thermus thermophilus |