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Literature summary for 2.7.7.7 extracted from
- Purification and properties of the 5'-3' exonuclease D190->A mutant of DNA polymerase I from Streptococcus pneumoniae (1998), Eur. J. Biochem., 252, 124-132.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and D10A mutant | Streptococcus pneumoniae |
| wild-type and D190A mutant | Streptococcus pneumoniae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D190A | retains polymerase activity, reduced exonuclease activity, changes in dependency on metal activation of exonuclease activity | Streptococcus pneumoniae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | exonuclease acitivity shows equal efficiency with Mg2+ and Mn2+, mutant D190A shows preference for Mn2+ | Streptococcus pneumoniae |  |
| Mn2+ | exonuclease acitivity shows equal efficiency with Mg2+ and Mn2+, mutant D190A shows preference for Mn2+ | Streptococcus pneumoniae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Streptococcus pneumoniae | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
D190A mutant | Streptococcus pneumoniae |
| 9.84 | - |
D10A mutant | Streptococcus pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + primed M13 | - |
Streptococcus pneumoniae | ? | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Streptococcus pneumoniae | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | exonuclease 5'--3' activity, pol I | Streptococcus pneumoniae | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + primed M13n | - |
Streptococcus pneumoniae | diphosphate + primed M13n+1 | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000042 | - |
primed M13 | exonuclease activity, D190A mutant, pH 7.6, 37°C | Streptococcus pneumoniae | |
| 0.011 | - |
primed M13 | exonuclease activity, wild-type, pH 7.6, 37°C | Streptococcus pneumoniae | |
| 0.056 | - |
ATP | polymerase activity, D190A mutant, pH 7.6, 37°C | Streptococcus pneumoniae | |
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