Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aphidicolin | 50% inhibition at 0.05 mM | [Bacillus] caldotenax | |
Ara-CTP | 50% inhibition at 0.05 mM | [Bacillus] caldotenax | |
Dideoxythymidine triphosphate | 50% inhibition at 0.25 mM | [Bacillus] caldotenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ in activation, optimal concentration: 2.5 mM | [Bacillus] caldotenax | |
K+ | stimulates at 100 mM | [Bacillus] caldotenax | |
Mg2+ | stimulates at 70 mM | [Bacillus] caldotenax | |
Mn2+ | can partially replace Mg2+ in activation | [Bacillus] caldotenax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
1 * 94000, SDS-PAGE | [Bacillus] caldotenax |
110000 | 120000 | HPLC gel filtration, non-denaturing PAGE | [Bacillus] caldotenax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | [Bacillus] caldotenax | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Bacillus] caldotenax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Bacillus] caldotenax |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
[Bacillus] caldotenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
[Bacillus] caldotenax | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | template specificity | [Bacillus] caldotenax | diphosphate + DNAn+1 | - |
? | |
dNTP + DNAn | - |
[Bacillus] caldotenax | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 94000, SDS-PAGE | [Bacillus] caldotenax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
[Bacillus] caldotenax |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 55 | 37°C: 15% of activity maximum, 55°C: 65% of activity maximum | [Bacillus] caldotenax |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
t1/2: 1.5 h without glycerol, 20 h in presence of glycerol | [Bacillus] caldotenax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
dNTP | - |
[Bacillus] caldotenax | |
1.05 | - |
deoxynucleoside triphosphate | equimolar mixture of all nucleotides, pH 7.5, 70°C | [Bacillus] caldotenax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
[Bacillus] caldotenax |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
[Bacillus] caldotenax | PAGE, pH range 3-9, pH 7.5, 70°C | - |
4.9 |