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Literature summary for 2.7.7.7 extracted from
- Purification and characterization of PRD1 DNA polymerase (1994), Biochim. Biophys. Acta, 1219, 267-276.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aphidicolin | - |
Enterobacteria phage PRD1 |  |
| dideoxyguanosine triphosphate | - |
Enterobacteria phage PRD1 | |
| K+ | 50% inhibition at 270 mM | Enterobacteria phage PRD1 | |
| KCl | - |
Enterobacteria phage PRD1 | |
| N-ethylmaleimide | - |
Enterobacteria phage PRD1 | |
| NaCl | - |
Enterobacteria phage PRD1 | |
| NH4Cl | - |
Enterobacteria phage PRD1 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | bacteriophage PRD1 enzyme overexpressed in Escherichia coli has a soluble and an insoluble activity indistinguishable by enzymatic properties | Enterobacteria phage PRD1 | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
two-dimensional electrophoresis, second dimension: SDS-PAGE | Enterobacteria phage PRD1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Enterobacteria phage PRD1 | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacteria phage PRD1 | - |
enzyme overexpressed in Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme overexpressed in Escherichia coli | Enterobacteria phage PRD1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Enterobacteria phage PRD1 | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' activity | Enterobacteria phage PRD1 | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | template specificity, enzyme overexpressed in E. coli | Enterobacteria phage PRD1 | diphosphate + DNAn+1 | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | 4 | deoxynucleoside triphosphate | pH 7.6, 30°C | Enterobacteria phage PRD1 | |
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