Application | Comment | Organism |
---|---|---|
biotechnology | thermostable polymerase used in PCR | Thermus aquaticus |
molecular biology | thermostable polymerase used in PCR | Thermus aquaticus |
Cloned (Comment) | Organism |
---|---|
wild-type and truncated form | Thermus aquaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates at 10-55 mM KCl | Thermus aquaticus | |
Mg2+ | required | Thermus aquaticus | |
Mg2+ | optimal concentration: 2-4 mM | Thermus aquaticus | |
Mg2+ | MgCl2 is the preferred cofactor compared to MnCl2, CoCl2 and NiCl2 | Thermus aquaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
gel filtration | Thermus aquaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Thermus aquaticus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
high-level expression | - |
Purification (Comment) | Organism |
---|---|
high-level expression | Thermus aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermus aquaticus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | wild-type enzyme, but not the truncated form has exonuclease 5'--3' activity | Thermus aquaticus | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 80 | - |
Thermus aquaticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
97.5 | - |
half-life: 9 min | Thermus aquaticus |