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Literature summary for 2.7.7.7 extracted from

  • Lawyer, F.C.; Stoffel, S.; Saiki, R.K.; Chang, S.Y.; Landre, P.A.; Abramson, R.D.; Gelfand, D.H.
    High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5 to 3 exonuclease activity (1993), PCR Methods Appl., 2, 275-287.
    View publication on PubMed

Application

Application Comment Organism
biotechnology thermostable polymerase used in PCR Thermus aquaticus
molecular biology thermostable polymerase used in PCR Thermus aquaticus

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and truncated form Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates at 10-55 mM KCl Thermus aquaticus
Mg2+ required Thermus aquaticus
Mg2+ optimal concentration: 2-4 mM Thermus aquaticus
Mg2+ MgCl2 is the preferred cofactor compared to MnCl2, CoCl2 and NiCl2 Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
gel filtration Thermus aquaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermus aquaticus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
high-level expression
-

Purification (Commentary)

Purification (Comment) Organism
high-level expression Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Thermus aquaticus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn wild-type enzyme, but not the truncated form has exonuclease 5'--3' activity Thermus aquaticus diphosphate + DNAn+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75 80
-
Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
97.5
-
half-life: 9 min Thermus aquaticus