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Literature summary for 2.7.7.7 extracted from

  • Campos, L.R.; Suarez, M.; Vazquez, A.; de la Fuente, R.; Herrera, L.
    Molecular cloning of the gene, expression in E. coli and purification of the Thermus aquaticus DNA polymerase I (1992), Acta Biotechnol., 12, 155-159.
No PubMed abstract available
Search for more literature for 2.7.7.7

Application

Application Comment Organism
biotechnology thermostable polymerase used in PCR Thermus aquaticus
molecular biology thermostable polymerase used in PCR Thermus aquaticus

Cloned(Commentary)

Cloned (Comment) Organism
polymerase I gene expressed in Escherichia coli Thermus aquaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermus aquaticus
-
 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn Thermus aquaticus YT1
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-
Thermus aquaticus YT1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polymerase I, expressed in Escherichia coli Thermus aquaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 polymerase I Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Thermus aquaticus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn
-
 Thermus aquaticus YT1 diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Thermus aquaticus