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Literature summary for 2.7.7.7 extracted from

  • Spampinato, C.P.; Pairoba, C.F.; Colombo, S.L.; Benediktsson, I.; Andreo, C.S.
    Properties of DNA polymerase from petunia "Mitchell" chloroplasts. Inhibitory effects of flavonoids (1994), Biosci. Biotechnol. Biochem., 58, 822-825.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2',3'-dideoxythymidine 5'-triphosphate
-
Ruellia sp.
alpha-Amanitin 88% inhibition at 0.2 mg/ml Ruellia sp.
aphidicolin
-
Ruellia sp.
Dideoxythymidine triphosphate
-
Ruellia sp.
ethanol 21% inhibition at 10% Ruellia sp.
flavonoids substitution of hydroxyl groups with glycosides Ruellia sp.
N-ethylmaleimide 59% inhibition at 1 mM Ruellia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Ruellia sp. 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates Ruellia sp.
Mg2+ required Ruellia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
SDS-PAGE Ruellia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Ruellia sp.
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Ruellia sp.
-
petunia, Mitchell
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Ruellia sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Ruellia sp. diphosphate + DNAn+1
-
?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Ruellia sp.