Cloned (Comment) | Organism |
---|---|
gene rpoA, recombinant expression of the HA-tagged alpha-subunit in transplastomic tobacco plants, analysis of the distribution of the tagged polymerase in plastid subfractions, and associated genes are identified under various light conditions. RpoA:HA is detected as early as the 3rd day after imbibition, and is constitutively expressed in green tissue over 60 days of plant development. Phenotypes of tagged RpoA and wild-type control lines, overview. Transplastomic tobacco plants grown on soil are phenotypically indistinguishable from wild-type | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | light-dependent, plastome-wide association of the plastid-encoded RNA polymerase with chloroplast DNA. The polymerase alpha-subunit preferentially associates with the plastid membranes, and is less abundant in the soluble stroma fraction | Nicotiana tabacum | 9507 | - |
chloroplast membrane | attachment of RpoA:HA to the membrane fraction during early seedling development is independent of DNA, but at later stages of development, DNA appears to facilitate attachment of the polymerase to membranes | Nicotiana tabacum | 31969 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | P06269 | gene rpoA encoding subunit A | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
additional information | spatio-temporal expression of plastid RNA polymerase: the enzyme is constitutively expressed in green tissue over 60 days of plant development | Nicotiana tabacum | - |
seedling | - |
Nicotiana tabacum | - |
Synonyms | Comment | Organism |
---|---|---|
plastid RNA polymerase | - |
Nicotiana tabacum |
plastid-encoded RNA polymerase | - |
Nicotiana tabacum |
RpoA | - |
Nicotiana tabacum |
General Information | Comment | Organism |
---|---|---|
physiological function | plastid genes are transcribed by two types of RNA polymerases: a plastid-encoded eubacterial-type RNA polymerase and nuclear-encoded phage-type RNA polymerases, spatio-temporal expression of plastid RNA polymerase. The association of plastid RNA polymerase with photosynthesis-related genes is reduced during the dark period, indicating that plastome-wide plastid RNA polymerase-DNA association is a light-dependent process | Nicotiana tabacum |