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Literature summary for 2.7.7.6 extracted from

  • Merkl, P.; Perez-Fernandez, J.; Pilsl, M.; Reiter, A.; Williams, L.; Gerber, J.; Boehm, M.; Deutzmann, R.; Griesenbeck, J.; Milkereit, P.; Tschochner, H.
    Binding of the termination factor Nsi1 to its cognate DNA site is sufficient to terminate RNA polymerase I transcription in vitro and to induce termination in vivo (2014), Mol. Cell. Biol., 34, 3817-3827.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.6

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type ProtA-tagged enzyme PolI in strain y2423. The second largest subunit of one polymerase is expressed as a C-terminal fusion protein with a protein A tag. Between the C terminus of the subunit and the protein A part, a recognition site for TEV protease is located Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
terminatin factor NsiI N-terminally FLAG-tagged fusion protein Nsi1 expressed from Sf21 insect cells. Binding of the termination factor Nsi1 to its cognate DNA site is sufficient to terminate RNA polymerase I transcription in vitro and to induce termination in vivo. Nsi1 contains Myb-like DNA binding domains and associates in vivo near the 3' end of rRNA genes to rDNA. Binding of Nsi1 to a stretch of 11 nucleotides in the correct orientation is sufficient to pause elongating Pol I shortly upstream of the Nsi1 binding site and to release the transcripts in vitro, and the same minimal DNA element triggers Nsi1-dependent termination of pre-rRNA synthesis in vivo. Termination efficiency in the in vivo system can be enhanced by inclusion of specific DNA sequences downstream of the Nsi1 binding site Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
-
Saccharomyces cerevisiae y2423
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type ProtA-tagged enzyme PolI from strain y2423 by protein A affinity chromatography and cleavage of the ProtA tag by TEV protease Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
polI
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 Saccharomyces cerevisiae
RNA polymerase I
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 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function binding of the termination factor Nsi1 to its cognate DNA site is sufficient to terminate RNA polymerase I transcription in vitro and to induce termination in vivo. Nsi1 contains Myb-like DNA binding domains and associates in vivo near the 3' end of rRNA genes to rDNA Saccharomyces cerevisiae