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Literature summary for 2.7.7.6 extracted from

  • Ho, T.H.; Hong, M.K.; Ngo, H.P.; Kang, L.W.
    Expression, crystallization and preliminary X-ray crystallographic analysis of DNA-directed RNA polymerase subunit L from Thermococcus onnurineus NA1 (2014), Acta Crystallogr. Sect. F, 70, 639-642.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene TON-0309 encoding DNA-directed RNA polymerase subunit L, recombinant overexpression of subunit L in Escherichia coli strain BL21(DE3) Thermococcus onnurineus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant DNA-directed RNA polymerase subunit L, hanging drop vapour diffusion method, mixing of 15 mg/ml protein in a buffer consisting of 25 mM Tris-HCl, pH 7.5, 15 mM NaCl, 3 mM 2-mercaptoethanol, with 500 nl reservoir solution containing 0.2 M lithium sulfate, 21% w/v PEG 3350, 15% v/v MPD, 0.1 M imidazole-HCl, pH 6.0, and equilibration against 0.07 ml of reservoir solution, 10-14°C, X-ray diffraction structure determination and analysis at 2.1 A resolution Thermococcus onnurineus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn Thermococcus onnurineus
-
diphosphate + RNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus onnurineus B6YTA7 gene TON-0309
-

Purification (Commentary)

Purification (Comment) Organism
recombinant RNAP subunit L from Escherichia coli strain BL21(DE3) by anion exchange chromatography and ultrafiltration Thermococcus onnurineus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
-
Thermococcus onnurineus diphosphate + RNAn+1
-
?

Synonyms

Synonyms Comment Organism
RNA polymerase
-
Thermococcus onnurineus
RNAP
-
Thermococcus onnurineus

General Information

General Information Comment Organism
physiological function RNA polymerase plays a crucial role in gene expression in all organisms. It is a multiprotein complex that produces primary transcript RNA from a DNA template Thermococcus onnurineus