Cloned (Comment) | Organism |
---|---|
gene TON-0309 encoding DNA-directed RNA polymerase subunit L, recombinant overexpression of subunit L in Escherichia coli strain BL21(DE3) | Thermococcus onnurineus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant DNA-directed RNA polymerase subunit L, hanging drop vapour diffusion method, mixing of 15 mg/ml protein in a buffer consisting of 25 mM Tris-HCl, pH 7.5, 15 mM NaCl, 3 mM 2-mercaptoethanol, with 500 nl reservoir solution containing 0.2 M lithium sulfate, 21% w/v PEG 3350, 15% v/v MPD, 0.1 M imidazole-HCl, pH 6.0, and equilibration against 0.07 ml of reservoir solution, 10-14°C, X-ray diffraction structure determination and analysis at 2.1 A resolution | Thermococcus onnurineus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | Thermococcus onnurineus | - |
diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus onnurineus | B6YTA7 | gene TON-0309 | - |
Purification (Comment) | Organism |
---|---|
recombinant RNAP subunit L from Escherichia coli strain BL21(DE3) by anion exchange chromatography and ultrafiltration | Thermococcus onnurineus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Thermococcus onnurineus | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA polymerase | - |
Thermococcus onnurineus |
RNAP | - |
Thermococcus onnurineus |
General Information | Comment | Organism |
---|---|---|
physiological function | RNA polymerase plays a crucial role in gene expression in all organisms. It is a multiprotein complex that produces primary transcript RNA from a DNA template | Thermococcus onnurineus |