Literature summary for 2.7.7.6 extracted from

Spahr, H.; Calero, G.; Bushnell, D.A.; Kornberg, R.D.
Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution (2009), Proc. Natl. Acad. Sci. USA, 106, 9185-9190.

Crystallization (Commentary)

Crystallization (Comment)	Organism
Pol II in complex with transcription factor TFIIB, sessile drop-vapor diffusion method, 11 mg/ml protein in 10 mM Tris-HCl, pH 7.5, 25 mM KCl, 0.1 mM EDTA, 0.001 mM ZnCl2, 10 mM DTT, and 14.2 mM pentaethyleneglycol monooctyl ether, is mixed with an equal volume of reservoir solution containing 50 mM MES, pH 6.3, 300 mM ammonium acetate, 5-6.5% w/v PEG 4000, and 10 mM DTT, 9 days at 16°C, crystals are crushed and microseeded into a fresh drop prepared as before except with MES, pH 6.6, and 3.6% PEG 4000. After several rounds of microseeding, long rod-shaped crystals are obtained, cryoprotection by 15-30% glycerol, X-ray difraction structure determination and analysis at 3.65 A resolution. molecular modelling	Schizosaccharomyces pombe

Crystallization (Comment)

Organism

Pol II in complex with transcription factor TFIIB, sessile drop-vapor diffusion method, 11 mg/ml protein in 10 mM Tris-HCl, pH 7.5, 25 mM KCl, 0.1 mM EDTA, 0.001 mM ZnCl2, 10 mM DTT, and 14.2 mM pentaethyleneglycol monooctyl ether, is mixed with an equal volume of reservoir solution containing 50 mM MES, pH 6.3, 300 mM ammonium acetate, 5-6.5% w/v PEG 4000, and 10 mM DTT, 9 days at 16°C, crystals are crushed and microseeded into a fresh drop prepared as before except with MES, pH 6.6, and 3.6% PEG 4000. After several rounds of microseeding, long rod-shaped crystals are obtained, cryoprotection by 15-30% glycerol, X-ray difraction structure determination and analysis at 3.65 A resolution. molecular modelling

Schizosaccharomyces pombe

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	P36594	-	-

Subunits

Subunits	Comment	Organism
More	enzyme structure analysis and comparison to the structures of Saccharomyces cerevisiae and human Pol IIs, overview	Schizosaccharomyces pombe

Synonyms

Synonyms	Comment	Organism
Pol II	-	Schizosaccharomyces pombe
RNA polymerase II	-	Schizosaccharomyces pombe

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)