Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)R3 Rosetta cells | Burkholderia pseudomallei |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6, 30% (w/v) PEG 2000 | Burkholderia pseudomallei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia pseudomallei | Q3JW91 | - |
- |
Purification (Comment) | Organism |
---|---|
His Trap column chromatography, and Superdex 200 gel filtration | Burkholderia pseudomallei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pantetheine 4'-phosphate | - |
Burkholderia pseudomallei | diphosphate + 3'-dephospho-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | x-ray crystallography | Burkholderia pseudomallei |
Synonyms | Comment | Organism |
---|---|---|
phosphopantetheine adenylyltransferase | - |
Burkholderia pseudomallei |
PPAT | - |
Burkholderia pseudomallei |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the fourth of five steps in the coenzyme A biosynthetic pathway | Burkholderia pseudomallei |