Literature summary for 2.7.7.3 extracted from

Timofeev, V.; Smirnova, E.; Chupova, L.; Esipov, R.; Kuranova, I.
X-ray study of the conformational changes in the molecule of phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis during the catalyzed reaction (2012), Acta Crystallogr. Sect. D, 68, 1660-1670.

Search for more literature for 2.7.7.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli ER2566 cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo form and in complex with ATP, counter-diffusion method, using 40 mM cacodylate buffer pH 5.5 containing 10 mM MgCl2, 0.15 mM NaCl, 20 mM cobalt hexamine and 15% (w/v) 2-methyl-2,4-pentanediol as precipitant solution	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WPA5	-	-
Mycobacterium tuberculosis H37Rv	P9WPA5	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q Sepharose XL resin column chromatography, Q Sepharose HP resin column chromatography, and Sepharose S-200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pantetheine 4'-phosphate	-	Mycobacterium tuberculosis	diphosphate + 3'-dephospho-CoA	-	r
ATP + pantetheine 4'-phosphate	-	Mycobacterium tuberculosis H37Rv	diphosphate + 3'-dephospho-CoA	-	r

Subunits

Subunits	Comment	Organism
hexamer	x-ray crystallography	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
phosphopantetheine adenylyltransferase	-	Mycobacterium tuberculosis
PPAT	-	Mycobacterium tuberculosis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)