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Literature summary for 2.7.7.2 extracted from

  • Sebastian, M.; Lira-Navarrete, E.; Serrano, A.; Marcuello, C.; Velazquez-Campoy, A.; Lostao, A.; Hurtado-Guerrero, R.; Medina, M.; Martinez-Julvez, M.
    The FAD synthetase from the human pathogen Streptococcus pneumoniae a bifunctional enzyme exhibiting activity-dependent redox requirements (2017), Sci. Rep., 7, 7609 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.2

Activating Compound

Activating Compound Comment Organism Structure
Dithionite presence of sodium dithionite is required for the transformation of FMN into FAD Streptococcus pneumoniae
dithiothreitol FMNAT activity is very poor in the presence of milder reducing agents such as DTT Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0098
-
 FMN pH 7.0, 25°C Streptococcus pneumoniae
0.0316
-
 ATP pH 7.0, 25°C Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximum activity at 0.8-1.2 mM Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + FMN
-
 Streptococcus pneumoniae diphosphate + FAD
-
 ?
additional information FADS catalyzes the activities of riboflavin kinase (RFK, EC 2.7.1.26), TP:FMN:adenylyltransferase (FMNAT), and FAD diphosphorylase (FADpp). The FMNAT and FADSpp activities require flavin substrates in the reduced state and binding of adenine nucleotide ligands is required for the binding of flavinic substrates/products. FADS does not bind oxidized flavins by itself Streptococcus pneumoniae ?
-
-

Subunits

Subunits Comment Organism
? x * 34800, calculated from sequence Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
SPAP_1083
-
 Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 ATP pH 7.0, 25°C Streptococcus pneumoniae
0.58
-
 FMN pH 7.0, 25°C Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.7
-
 ATP pH 7.0, 25°C Streptococcus pneumoniae
60
-
 FMN pH 7.0, 25°C Streptococcus pneumoniae