Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of bovine residue N202 (equivalent to yeast N189) to alanine has essentially no effect on the apparent Km for ATP, but has a pronounced effect on the apparent Vmax, suggesting that this residue is particularly important in the recognition of the adenine of ATP | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
a decay of PAP activity with a half-life of approximately 2 h (on ice) is observed when Nonidet-P 40 is omitted from the enzyme storage solution | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha, beta-methylene-ATP | a nonreactive ATP analogue | Saccharomyces cerevisiae | |
diphosphate | product inhibitor | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0263 | - |
oligo(A)17C | incubation at 30 °C for 15 min in the presence of MgATP2- | Saccharomyces cerevisiae | |
0.0359 | - |
ATP | - |
Saccharomyces cerevisiae | |
0.0468 | - |
oligo(A)18 | incubation at 30 °C for 15 min in the presence of MgATP2- | Saccharomyces cerevisiae | |
0.0642 | - |
oligo(A)18 | incubation at 30 °C for 15 min in the presence of MgCTP2- | Saccharomyces cerevisiae | |
0.1036 | - |
CTP | - |
Saccharomyces cerevisiae | |
0.123 | - |
ATP | incubation at 30 °C for 15 min, using oligo(A)17C (a modification of the 3'-terminal residue from A to C) | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant yeast PAP is purified by nickel affinity and anion exchange chromatography using NTA agarose and Source S resins | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oligo(A)17C | - |
Saccharomyces cerevisiae | diphosphate + oligo(A)18C | - |
? | |
ATP + oligo(A)18 | - |
Saccharomyces cerevisiae | diphosphate + oligo(A)19 | - |
? | |
ATP + RNAn | - |
Saccharomyces cerevisiae | diphosphate + RNAn+1 | - |
? | |
CTP + RNAn | - |
Saccharomyces cerevisiae | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Saccharomyces cerevisiae |
polyA polymerase | - |
Saccharomyces cerevisiae |
polyadenylate polymerase | - |
Saccharomyces cerevisiae |