Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | major poly(A) polymerase, optimal at 250 mM, minor poly(A) polymerase, optimal at 125 mM, inhibition of both above 250 mM | Bacillus subtilis | |
spermidine | slight stimulation, 5 mM | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 28% inhibition, major poly(A) polymerase, 50% inhibition, minor poly(A) polymerase | Bacillus subtilis | |
cordycepin | not | Bacillus subtilis | |
CTP | - |
Bacillus subtilis | |
dATP | 0.25 mM, 50% inhibition, major poly(A) polymerase, 15% inhibition, minor poly(A) polymerase | Bacillus subtilis | |
GTP | - |
Bacillus subtilis | |
KCl | stimulation at low concentrations, at 300 mM, 15% inhibition, major poly(A) polymerase, 52% inhibition, minor poly(A) polymerase | Bacillus subtilis | |
additional information | not inhibitory: rifampicin, streptolydigin, phosphate at 0.5 mM, cordycepin at 0.1 mM | Bacillus subtilis | |
PO43- | not inhibitory | Bacillus subtilis | |
rifampicin | not inhibitory | Bacillus subtilis | |
UTP | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.143 | - |
ATP | major poly(A) polymerase, 37°C, pH 8.0 | Bacillus subtilis | |
0.4 | - |
ATP | major poly(A) polymerase, 37°C, pH 8.0 | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bacillus subtilis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | more active in presence of Mn2+ than Mg2+ | Bacillus subtilis | |
Mn2+ | more active in presence of Mn2+ than Mg2+ | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration, minor poly(A) polymerase | Bacillus subtilis |
86000 | - |
gel filtration, major poly(A) polymerase | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
two isoforms | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | primer required | Bacillus subtilis | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | enzyme also has cleavage activity | Bacillus subtilis | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer: poly(A) | Bacillus subtilis | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer: dephosphorylated poly(A), tRNA | Bacillus subtilis | diphosphate + RNA(A)n | - |
? |