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Literature summary for 2.7.7.19 extracted from
- A kinetic and structural characterization of adenosine-5-triphosphate: ribonucleic acid adenylyltransferase from Pseudomonas putida (1975), Biochim. Biophys. Acta, 414, 133-145.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-dATP | - |
Homo sapiens |  |
| 3'-dATP | - |
Pseudomonas putida | |
| cordycepin | - |
Pseudomonas putida | |
| diphosphate | - |
Homo sapiens | |
| diphosphate | - |
Pseudomonas putida | |
| Poly(dT) | - |
Pseudomonas putida | |
| Poly(U) | - |
Pseudomonas putida | |
| Rifamycin derivatives | some derivatives are effective, others not | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | - |
Pseudomonas putida |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | - |
Pseudomonas putida | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | more active in presence of Mg2+ than Mn2+ | Pseudomonas putida | |
| Mn2+ | - |
Homo sapiens | |
| Mn2+ | more active in presence of Mg2+ than Mn2+ | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
- |
Pseudomonas putida |
| 58000 | - |
adenylyltransferase B, glycerol density gradient sedimentation | Homo sapiens |
| 185000 | - |
adenylyltransferase A, glycerol density gradient sedimentation | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| adenylyltransferase A and B | Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 3 months | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RNA | primer: mixture of tRNA | Pseudomonas putida | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | primer required | Homo sapiens | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | primer required | Pseudomonas putida | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | poly(A) and poly(C) minimally effective | Pseudomonas putida | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | no primer: poly(dT) | Pseudomonas putida | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | primer: oligonucleotides A-A-A-A and A-A-A | Pseudomonas putida | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | no primer: poly(U) | Pseudomonas putida | diphosphate + RNA(A)n | - |
? | |
| CTP + RNA | 12% of the activity with ATP, adenylyltransferase A | Pseudomonas putida | diphosphate + ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
adenylyltransferase A | Pseudomonas putida |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | 1 | 3'-dATP | - |
Pseudomonas putida | |
| 4 | 10 | Poly(U) | - |
Pseudomonas putida | |
| 4 | 10 | Poly(dT) | - |
Pseudomonas putida | |
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