Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | 2 forms: one from chromatin and one from nucleoplasm | Rattus norvegicus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration: 8-10 mM, polymerase I from chromatin, polymerase II from nucleoplasm is inactive in presence of Mg2+ | Rattus norvegicus | |
Mn2+ | optimal concentration: 0.8 mM (polymerase I and II) | Rattus norvegicus | |
additional information | low ionic strength required for maximal activity | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | Rattus norvegicus | 2 enzymes participate in the polyadenylation of chromosomal RNA, by a coupled mechanism, the chromatin bound enzyme adds 120-130 adenosine nucleotides to chromosomal RNA, the nucleoplasmic enzyme completes the polyadenylation by adding 80-90 more AMP units to the polyadenylated end | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2 forms, one from chromatin and one from nucleoplasm | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | highly specific for ATP | Rattus norvegicus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | chromatin enzyme uses chromosomal RNA as primer, enzyme from nucleoplasm uses poly(A) and hnRNA isolated from chromatin as primer | Rattus norvegicus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer required | Rattus norvegicus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | 2 enzymes participate in the polyadenylation of chromosomal RNA, by a coupled mechanism, the chromatin bound enzyme adds 120-130 adenosine nucleotides to chromosomal RNA, the nucleoplasmic enzyme completes the polyadenylation by adding 80-90 more AMP units to the polyadenylated end | Rattus norvegicus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
polymerase II (nucleoplasm) | Rattus norvegicus |
8.5 | - |
polymerase I (chromatin) | Rattus norvegicus |