KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
poly(A)n | Mn2+-activated enzyme, pH 8.3, 37°C | Bos taurus | |
0.01 | - |
oligo(A)n | Mn2+-activated enzyme, pH 8.3, 37°C | Bos taurus | |
0.14 | 0.36 | poly(A)n | Mg2+-activated enzyme, pH 8.3, 37°C | Bos taurus | |
0.3 | - |
oligo(A)n | Mg2+-activated enzyme, pH 8.3, 37°C | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | requirement is dependent on the primer and the divalent cation used | Bos taurus | |
Mg2+ | more active in presence of Mn2+ than Mg2+ | Bos taurus | |
Mg2+ | optimal concentration: 4-6 mM | Bos taurus | |
Mn2+ | more active in presence of Mn2+ than Mg2+ | Bos taurus | |
Mn2+ | optimal concentration: 0.5 mM (at 0.5 mM ATP) | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
and 60000, 2 major forms of enzyme, gel filtration | Bos taurus |
60000 | - |
and 57000, 2 major forms of enzyme, gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | Bos taurus | involved in the 3'-end processing of mRNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | not glycosylated | Bos taurus |
Purification (Comment) | Organism |
---|---|
calf | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-80°C, storage for 5 days including 2 cycles of freezing and thawing results in 35% inactivation | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oligo(A)n | - |
Bos taurus | diphosphate + oligo(A)n+1 | - |
? | |
ATP + poly(A)n | - |
Bos taurus | diphosphate + poly(A)n+1 | - |
? | |
ATP + RNA | elongation of the primer is distributive | Bos taurus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer required | Bos taurus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | involved in the 3'-end processing of mRNA | Bos taurus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
Mg2+-activated enzyme | Bos taurus |
8.3 | - |
Mn2+-activated enzyme | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.2 | pH 7.2: 55% of maximum activity, pH 9.2: 62% of maximum activity | Bos taurus |