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Literature summary for 2.7.7.19 extracted from

  • Wahle, E.
    Purification and characterization of a mammalian polyadenylate polymerase involved in the 3 end processing of messenger RNA precursors (1991), J. Biol. Chem., 266, 3131-3139.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
poly(A)n Mn2+-activated enzyme, pH 8.3, 37°C Bos taurus
0.01
-
oligo(A)n Mn2+-activated enzyme, pH 8.3, 37°C Bos taurus
0.14 0.36 poly(A)n Mg2+-activated enzyme, pH 8.3, 37°C Bos taurus
0.3
-
oligo(A)n Mg2+-activated enzyme, pH 8.3, 37°C Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl requirement is dependent on the primer and the divalent cation used Bos taurus
Mg2+ more active in presence of Mn2+ than Mg2+ Bos taurus
Mg2+ optimal concentration: 4-6 mM Bos taurus
Mn2+ more active in presence of Mn2+ than Mg2+ Bos taurus
Mn2+ optimal concentration: 0.5 mM (at 0.5 mM ATP) Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
and 60000, 2 major forms of enzyme, gel filtration Bos taurus
60000
-
and 57000, 2 major forms of enzyme, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + RNA Bos taurus involved in the 3'-end processing of mRNA ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information not glycosylated Bos taurus

Purification (Commentary)

Purification (Comment) Organism
calf Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-80°C, storage for 5 days including 2 cycles of freezing and thawing results in 35% inactivation Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oligo(A)n
-
Bos taurus diphosphate + oligo(A)n+1
-
?
ATP + poly(A)n
-
Bos taurus diphosphate + poly(A)n+1
-
?
ATP + RNA elongation of the primer is distributive Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA primer required Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA involved in the 3'-end processing of mRNA Bos taurus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
Mg2+-activated enzyme Bos taurus
8.3
-
Mn2+-activated enzyme Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9.2 pH 7.2: 55% of maximum activity, pH 9.2: 62% of maximum activity Bos taurus