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Literature summary for 2.7.7.19 extracted from

  • Pellicer, A.; Salas, J.; Salas, M.L.
    Characterization of two poly(A) polymerases from cultured hamster fibroblasts (1978), Biochim. Biophys. Acta, 519, 149-162.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3'-dATP
-
Mesocricetus auratus
CTP
-
Mesocricetus auratus
diphosphate
-
Mesocricetus auratus
GTP
-
Mesocricetus auratus
K+ above 50 mM; KCl Mesocricetus auratus
additional information not inhibitory: alpha-amanitin Mesocricetus auratus
N-ethylmaleimide
-
Mesocricetus auratus
UTP
-
Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ATP polymerase IIa, 37°C Mesocricetus auratus
0.05
-
ATP polymerase IIb, 37°C Mesocricetus auratus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ is inactive, maximum activity in presence of both Mn2+ and Mg2+ Mesocricetus auratus
Mn2+ maximum activity in presence of both Mn2+ and Mg2+ Mesocricetus auratus
Mn2+ optimal concentration: 4 mM (polymerase IIa), 4-8 mM (polymerase IIb) Mesocricetus auratus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
1 * 85000 + 1 * 60000, SDS-PAGE Mesocricetus auratus
85000
-
1 * 85000 + 1 * 60000, SDS-PAGE Mesocricetus auratus
145000
-
polymerase IIa, gel filtration Mesocricetus auratus
155000
-
polymerase IIb, gel filtration Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
CHO fibroblasts
-

Purification (Commentary)

Purification (Comment) Organism
polymerase IIa and IIb Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA highly specific for ATP Mesocricetus auratus diphosphate + RNA(A)n polymerase IIa: chain length of the product synthesized is independent of the primer concentration, polymerase IIb: the length of the product decreases when RNA concentration increases ?
ATP + RNA primer required Mesocricetus auratus diphosphate + RNA(A)n polymerase IIa: chain length of the product synthesized is independent of the primer concentration, polymerase IIb: the length of the product decreases when RNA concentration increases ?
ATP + RNA polymerase IIa and IIb utilize a variety of natural and synthetic RNAs as well as DNA as primer Mesocricetus auratus diphosphate + RNA(A)n polymerase IIa: chain length of the product synthesized is independent of the primer concentration, polymerase IIb: the length of the product decreases when RNA concentration increases ?

Subunits

Subunits Comment Organism
dimer 1 * 85000 + 1 * 60000, SDS-PAGE Mesocricetus auratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
polymerase IIa and IIb Mesocricetus auratus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6: 47% (polymerase IIa) and 5% (polymerase IIb) of maximum activity, pH 9: 55% of maximum activity Mesocricetus auratus