Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | infected cytoplasm of HeLa cells | Homo sapiens | 5737 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | HeLa cells contain one enzyme form that is stimulated by Mn2+ and also by Mg2+, and a second one that is absolutely dependent on the presence of Mg2+ | Homo sapiens | |
Mg2+ | Vaccinia virus enzyme is stimulated by Mn2+ and also by Mg2+ | Vaccinia virus | |
Mn2+ | HeLa cells contain one enzyme form that is stimulated by Mn2+ and also by Mg2+, and a second one that is absolutely dependent on the presence of Mg2+ | Homo sapiens | |
Mn2+ | Vaccinia virus enzyme is stimulated by Mn2+ and also by Mg2+ | Vaccinia virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
1 * 37000 + 1 * 57000, SDS-PAGE | Vaccinia virus |
50000 | - |
1 * 50000, nuclear Mn2+- and Mg2+-activated enzyme, SDS-PAGE | Homo sapiens |
57000 | - |
1 * 37000 + 1 * 57000, SDS-PAGE | Vaccinia virus |
58000 | - |
nuclear Mg2+- and Mn2+-stimulated enzyme, glycerol density gradient sedimentation | Homo sapiens |
63000 | - |
cytoplasmic Mn2+-dependent enzyme, glycerol density gradient sedimentation | Homo sapiens |
70000 | - |
enzyme from infected cytoplasm, glycerol density gradient sedimentation | Vaccinia virus |
75000 | - |
1 * 75000, cytoplasmic, Mn2+-dependent enzyme, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
HeLa infected with vaccinia virus | - |
Homo sapiens | - |
2 forms: 1. nuclear enzyme, stimulated by Mn2+ and Mg2+, 2. cytoplasmic, dependent on Mn2+ | - |
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Vaccinia virus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | primer required | Homo sapiens | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer required | Vaccinia virus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | human nuclear enzyme and Vaccinia virus enzyme are able to use both RNA and oligo(A) as primer, human cytoplasmic enzyme is able to use RNA but not oligo(A) | Homo sapiens | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | human nuclear enzyme and Vaccinia virus enzyme are able to use both RNA and oligo(A) as primer, human cytoplasmic enzyme is able to use RNA but not oligo(A) | Vaccinia virus | diphosphate + RNA(A)n | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 37000 + 1 * 57000, SDS-PAGE | Vaccinia virus |
monomer | 1 * 75000, cytoplasmic, Mn2+-dependent enzyme, SDS-PAGE | Homo sapiens |
monomer | 1 * 50000, nuclear Mn2+- and Mg2+-activated enzyme, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
vaccinia virus enzyme, human, cytoplasmic Mn2+-dependent enzyme | Homo sapiens |
8 | - |
vaccinia virus enzyme, human, cytoplasmic Mn2+-dependent enzyme | Vaccinia virus |
8.3 | - |
human nuclear Mn2+- and Mg2+-activated enzyme | Homo sapiens |