Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-Amanitin | - |
Rattus norvegicus | |
ATP | hepatoma enzyme less effective to substrate inhibition than liver enzyme | Rattus norvegicus | |
Cordycepin 5'-triphosphate | - |
Rattus norvegicus | |
diphosphate | - |
Rattus norvegicus | |
K+ | KCl; maximal stimulation at 40 mM, inhibition above 250 mM | Rattus norvegicus | |
N-ethylmaleimide | - |
Rattus norvegicus | |
Pancreatic ribonuclease | - |
Rattus norvegicus | |
Rose bengal | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ATP | 37°C, pH 8.0 | Rattus norvegicus | |
0.15 | - |
ATP | 37°C, pH 8.0 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | maximal stimulation at 40 mM, inhibition above 250 mM | Rattus norvegicus | |
Mg2+ | 10% of the activity with Mn2+ | Rattus norvegicus | |
Mn2+ | absolute requirement | Rattus norvegicus | |
Mn2+ | optimal concentration: 0.25-0.75 mM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, liver enzyme, SDS-PAGE | Rattus norvegicus |
60000 | - |
x * 60000, hepatoma enzyme, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatoma cell | Morris hepatoma tumor cells 3924A | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | highly specific for ATP | Rattus norvegicus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer required | Rattus norvegicus | diphosphate + RNA(A)n | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, hepatoma enzyme, SDS-PAGE | Rattus norvegicus |
? | x * 48000, liver enzyme, SDS-PAGE | Rattus norvegicus |