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Literature summary for 2.7.7.19 extracted from

  • Bollum, F.J.; Chang, L.M.S.; Tsiapalis, C.M.; Dorson, J.W.
    Nucleotide polymerizing enzymes from calf thymus gland (1974), Methods Enzymol., 29E, 70-81.
No PubMed abstract available
Search for more literature for 2.7.7.19

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ one-fifth of the activity of Mg2+ in NTP activation Bos taurus
Mn2+ required for NTP activation Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
-
 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
homogenous preparation of bovine enzyme Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA highly specific for ATP Bos taurus diphosphate + RNA(A)n
-
 ?
ATP + RNA primer: viral RNA MS-2 and QB Bos taurus diphosphate + RNA(A)n
-
 ?
ATP + RNA primer required Bos taurus diphosphate + RNA(A)n
-
 ?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Bos taurus diphosphate + RNA(A)n
-
 ?
ATP + RNA primer: rRNA 16S, E. coli Bos taurus diphosphate + RNA(A)n
-
 ?
ATP + RNA primer: rRNA 23S, E. coli Bos taurus diphosphate + RNA(A)n
-
 ?
ATP + RNA primer: poly(A) Bos taurus diphosphate + RNA(A)n
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Bos taurus