Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Francisella tularensis |
Crystallization (Comment) | Organism |
---|---|
apoenzyme and in complex with substrate 6-hydroxymethyl-7,8-dihydropteridine or 2-(7-amino-1-methyl-4,5-dioxo-1,4,5,6-tetrahydorpyrimido[4,5-c]pyridazin-3-yl)propanoic acid, sitting drop vapor diffusion method, using 90 mM Tris (pH 8.0), 190 mM sodium acetate, 24% (w/v) polyethylene glycol (PEG) 4000, and 17% (v/v) glycerol, at 18°C | Francisella tularensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains two Mg2+ ions | Francisella tularensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50509 | - |
1 * 50509, the bifunctional enzyme HPPK-DHPS exists mainly as a monomer in solution, calculated from amino acid sequence | Francisella tularensis |
53000 | - |
1 * 53000, the bifunctional enzyme HPPK-DHPS exists mainly as a monomer in solution, sedimentation velocity and equilibrium analysis | Francisella tularensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Francisella tularensis | A0A6B2JKF9 | - |
- |
Francisella tularensis LVS | A0A6B2JKF9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Francisella tularensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 6-hydroxymethyl-7,8-dihydropteridine | - |
Francisella tularensis | AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate | - |
ir | |
ATP + 6-hydroxymethyl-7,8-dihydropteridine | - |
Francisella tularensis LVS | AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate | - |
ir | |
additional information | the enzyme does not hydrolyze alpha,beta-methyleneadenosine 5-triphosphate | Francisella tularensis | ? | - |
? | |
additional information | the enzyme does not hydrolyze alpha,beta-methyleneadenosine 5-triphosphate | Francisella tularensis LVS | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50509, the bifunctional enzyme HPPK-DHPS exists mainly as a monomer in solution, calculated from amino acid sequence | Francisella tularensis |
monomer | 1 * 53000, the bifunctional enzyme HPPK-DHPS exists mainly as a monomer in solution, sedimentation velocity and equilibrium analysis | Francisella tularensis |
Synonyms | Comment | Organism |
---|---|---|
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase | HPPK-DHPS, bifunctional enzyme | Francisella tularensis |
HPPK | - |
Francisella tularensis |