Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion sitting or hanging drop method. The protein is co-crystallised in the presence of AMP and D-ribose 5-phosphate, but in the electron density map of the active site only AMP and a sulphate ion are observed. Sulphate ion, reminiscent of the ammonium sulfate precipitation step of the purification, seems to bind tightly and, therefore, presumably occupies and blocks the D-ribose 5-phosphate binding site. The structure is determined at 2.8 A resolution | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Saccharolobus solfataricus | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
ATP + D-ribose 5-phosphate | Saccharolobus solfataricus P2 | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97Z86 | - |
- |
Saccharolobus solfataricus P2 | Q97Z86 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Saccharolobus solfataricus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
ATP + D-ribose 5-phosphate | - |
Saccharolobus solfataricus P2 | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
5-phosphoribosyl-1-alpha-diphosphate synthase | - |
Saccharolobus solfataricus |
PRPP synthase | - |
Saccharolobus solfataricus |
SSO1045 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Saccharolobus solfataricus |