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Literature summary for 2.7.6.1 extracted from

  • Breda, A.; Martinelli, L.K.; Bizarro, C.V.; Rosado, L.A.; Borges, C.B.; Santos, D.S.; Basso, L.A.
    Wild-type phosphoribosylpyrophosphate synthase (PRS) from Mycobacterium tuberculosis: a bacterial class II PRS? (2012), PLoS ONE, 7, e39245.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.6.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Mycobacterium tuberculosis
D-ribose 5-phosphate
-
 Mycobacterium tuberculosis
GDP
-
 Mycobacterium tuberculosis
UMP
-
 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 D-ribose 5-phosphate cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis
0.025
-
 ATP in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis Rv1017c
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.028
-
 crude extract, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis
1.16
-
 after 41.4fold purification, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
-80°C, 50 mM Tris-HCl (pH 7.5), up to 7 months, no loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
ATP + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis Rv1017c AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
CTP + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
CTP + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis Rv1017c CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
GTP + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
GTP + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis Rv1017c GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
UTP + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
UTP + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis Rv1017c UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer the enzyme predominates as a hexamer, glutaraldehyde cross-linking Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
phosphoribosylpyrophosphate synthase
-
 Mycobacterium tuberculosis
PRS
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.66
-
 ATP in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis
60.68
-
 D-ribose 5-phosphate cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.211
-
 D-ribose 5-phosphate cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.07
-
 in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis ADP
0.9
-
 in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis GDP
3
-
 in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis UMP

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
26
-
 ATP in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis
59
-
 D-ribose 5-phosphate cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis