Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(AI) cells | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
A181D | the mutant shows reduced specific activity compared to the wild type enzyme | Salmonella enterica |
D224A | the mutant shows reduced specific activity compared to the wild type enzyme | Salmonella enterica |
E306K | the mutant shows reduced specific activity compared to the wild type enzyme | Salmonella enterica |
G209V | the mutant shows 45fold reduced specific activity compared to the wild type enzyme | Salmonella enterica |
G50D | the mutant shows reduced specific activity compared to the wild type enzyme | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Salmonella enterica DM10373 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ resin column chromatography | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.016 | - |
mutant enzyme G209V, at pH 7.5 and 37°C | Salmonella enterica |
0.027 | - |
mutant enzyme D224A, at pH 7.5 and 37°C | Salmonella enterica |
0.032 | - |
mutant enzyme G50D, at pH 7.5 and 37°C | Salmonella enterica |
0.041 | - |
mutant enzyme E306K, at pH 7.5 and 37°C | Salmonella enterica |
0.054 | - |
mutant enzyme A181D, at pH 7.5 and 37°C | Salmonella enterica |
0.094 | - |
wild type enzyme, at pH 7.5 and 37°C | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Salmonella enterica | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
ir | |
ATP + D-ribose 5-phosphate | - |
Salmonella enterica DM10373 | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
phosphoribosylpyrophosphate synthetase | - |
Salmonella enterica |
PRPP synthetase | - |
Salmonella enterica |
PrsA | - |
Salmonella enterica |