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Literature summary for 2.7.6.1 extracted from

  • Koenigsknecht, M.; Fenlon, L.; Downs, D.
    Phosphoribosylpyrophosphate synthetase (PrsA) variants alter cellular pools of ribose 5-phosphate and influence thiamine synthesis in Salmonella enterica (2010), Microbiology, 156, 950-959.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(AI) cells Salmonella enterica

Protein Variants

Protein Variants Comment Organism
A181D the mutant shows reduced specific activity compared to the wild type enzyme Salmonella enterica
D224A the mutant shows reduced specific activity compared to the wild type enzyme Salmonella enterica
E306K the mutant shows reduced specific activity compared to the wild type enzyme Salmonella enterica
G209V the mutant shows 45fold reduced specific activity compared to the wild type enzyme Salmonella enterica
G50D the mutant shows reduced specific activity compared to the wild type enzyme Salmonella enterica

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-
Salmonella enterica DM10373
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ resin column chromatography Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.016
-
mutant enzyme G209V, at pH 7.5 and 37°C Salmonella enterica
0.027
-
mutant enzyme D224A, at pH 7.5 and 37°C Salmonella enterica
0.032
-
mutant enzyme G50D, at pH 7.5 and 37°C Salmonella enterica
0.041
-
mutant enzyme E306K, at pH 7.5 and 37°C Salmonella enterica
0.054
-
mutant enzyme A181D, at pH 7.5 and 37°C Salmonella enterica
0.094
-
wild type enzyme, at pH 7.5 and 37°C Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Salmonella enterica AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
ir
ATP + D-ribose 5-phosphate
-
Salmonella enterica DM10373 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
ir

Synonyms

Synonyms Comment Organism
phosphoribosylpyrophosphate synthetase
-
Salmonella enterica
PRPP synthetase
-
Salmonella enterica
PrsA
-
Salmonella enterica