Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.6.1 extracted from

  • Willemoes, M.; Hove-Jensen, B.; Larsen, S.
    Steady state kinetic model for the binding of substrates and allosteric effectors to Escherichia coli phosphoribosyl-diphosphate synthase (2000), J. Biol. Chem., 275, 35408-35412.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP nonlinear competitive inhibition Escherichia coli
Ca2+
-
Escherichia coli
D-ribose 5-phosphate at unsaturating phosphate concentrations Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
Mg2+ pH 8.0, 37ºC, phosphate 50 mM Escherichia coli
0.059
-
Mg2+ pH 8.0, 37ºC, phosphate 5 mM Escherichia coli
0.19
-
D-ribose 5-phosphate pH 8.0, 37ºC, phosphate 50 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ saturation with free Mg2+ results in hyperbolic activation by phosphate. At unsaturating free Mg2+ concentration, cooperative activation by phosphate is observed Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-ribose 5-phosphate Escherichia coli
-
AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Storage Stability

Storage Stability Organism
stored and diluted in the presence of phosphate 50 mM to maintain stability. With 2 mM MgATP2- or more, the enzyme is fully stable upon dilution and subsequent incubation at 37ºC Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Escherichia coli AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.032
-
Ca2+ pH 8.0, 37ºC, D-ribose 5-phosphate 5 mM, Kis value Escherichia coli
0.047
-
Ca2+ pH 8.0, 37ºC, D-ribose 5-phosphate 5 mM, Kii value Escherichia coli
0.053
-
Ca2+ pH 8.0, 37ºC, D-ribose 5-phosphate 0.5 mM, Kis value Escherichia coli
0.11
-
Ca2+ pH 8.0, 37ºC, D-ribose 5-phosphate 0.5 mM, Kii value Escherichia coli
0.334
-
ADP pH 8.0, 37ºC, D-ribose 5-phosphate 0.5 mM, Kii value Escherichia coli
0.716
-
D-ribose 5-phosphate pH 8.0, 37ºC, Kii value Escherichia coli
1.18
-
D-ribose 5-phosphate pH 8.0, 37ºC, Kis value Escherichia coli