Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | nonlinear competitive inhibition | Escherichia coli | |
Ca2+ | - |
Escherichia coli | |
D-ribose 5-phosphate | at unsaturating phosphate concentrations | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
Mg2+ | pH 8.0, 37ºC, phosphate 50 mM | Escherichia coli | |
0.059 | - |
Mg2+ | pH 8.0, 37ºC, phosphate 5 mM | Escherichia coli | |
0.19 | - |
D-ribose 5-phosphate | pH 8.0, 37ºC, phosphate 50 mM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | saturation with free Mg2+ results in hyperbolic activation by phosphate. At unsaturating free Mg2+ concentration, cooperative activation by phosphate is observed | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Escherichia coli | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Storage Stability | Organism |
---|---|
stored and diluted in the presence of phosphate 50 mM to maintain stability. With 2 mM MgATP2- or more, the enzyme is fully stable upon dilution and subsequent incubation at 37ºC | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Escherichia coli | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
Ca2+ | pH 8.0, 37ºC, D-ribose 5-phosphate 5 mM, Kis value | Escherichia coli | |
0.047 | - |
Ca2+ | pH 8.0, 37ºC, D-ribose 5-phosphate 5 mM, Kii value | Escherichia coli | |
0.053 | - |
Ca2+ | pH 8.0, 37ºC, D-ribose 5-phosphate 0.5 mM, Kis value | Escherichia coli | |
0.11 | - |
Ca2+ | pH 8.0, 37ºC, D-ribose 5-phosphate 0.5 mM, Kii value | Escherichia coli | |
0.334 | - |
ADP | pH 8.0, 37ºC, D-ribose 5-phosphate 0.5 mM, Kii value | Escherichia coli | |
0.716 | - |
D-ribose 5-phosphate | pH 8.0, 37ºC, Kii value | Escherichia coli | |
1.18 | - |
D-ribose 5-phosphate | pH 8.0, 37ºC, Kis value | Escherichia coli |