Crystallization (Comment) | Organism |
---|---|
in complex with phosphorylated N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-thymine and ADP, vapor diffusion method, using 14% (w/v) PEG 3350, 0.1 M HEPES pH 7.5, at 20°C | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5-bromo-dUMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.02 | - |
dTMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.02 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.024 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.1 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.13 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.15 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.16 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-bromouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.17 | - |
dUMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
1 | - |
N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
1 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-fluorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
1.7 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-uracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
1.9 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-fluorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
2.5 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-uracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dTMP | Homo sapiens | - |
ADP + dTDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23919 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3'-azido-2',3'-dideoxythymidine monophosphate | - |
Homo sapiens | ADP + 3'-azido-2',3'-dideoxythymidine diphosphate | - |
? | |
ATP + 5-bromo-dUMP | - |
Homo sapiens | ADP + 5-bromo-dUDP | - |
? | |
ATP + dTMP | - |
Homo sapiens | ADP + dTDP | - |
? | |
ATP + dUMP | - |
Homo sapiens | ADP + dUDP | - |
? | |
ATP + N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-thymine | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-fluorouracil | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-thymine | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-uracil | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-bromouracil | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-chlorouracil | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-fluorouracil | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-thymine | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-uracil | - |
Homo sapiens | ADP + ? | - |
? | |
additional information | no activity with cidofovir, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-uracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-chlorouracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-bromouracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-fluoro-uracil, and N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-thymine | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thymidine monophosphate kinase | - |
Homo sapiens |
thymidylate kinase | - |
Homo sapiens |
TMP kinase | - |
Homo sapiens |
TMPK | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-bromouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.074 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-uracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.09 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-fluorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.1 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.32 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.48 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-uracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.5 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.6 | - |
N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.73 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-fluorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.84 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.9 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
1.2 | - |
5-bromo-dUMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
3 | - |
dTMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
4.8 | - |
dUMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-uracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.047 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-fluorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.28 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-uracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.36 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-bromouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.6 | - |
N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.66 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
0.73 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-fluorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
3.2 | - |
N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
4.1 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
28 | - |
dUMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
35 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-thymine | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
45 | - |
N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
120 | - |
5-bromo-dUMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens | |
150 | - |
dTMP | at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 | Homo sapiens |