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Literature summary for 2.7.4.8 extracted from

  • Mori, S.; Tezuka, Y.; Arakawa, A.; Handa, N.; Shirouzu, M.; Akiyama, T.; Yokoyama, S.
    Crystal structure of the guanylate kinase domain from discs large homolog 1 (DLG1/SAP97) (2013), Biochem. Biophys. Res. Commun., 435, 334-338.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged GK domain fragment of human DLG1 in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified the GK domain fragment of human DLG1, hanging drop vapor diffusion method, mixing of 6.5 mg/ml protein in 20 mM Tris-HCl buffer, pH 8.0, 150 mM NaCl and 2 mM dithiothreitol, with reservoir solution containing 0.2 M sodium thiocyanate and 22% PEG 3350, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement and selenomethionine single-wavelength anomalous dispersion, using the PSD-95 GK domain, PDB ID 1JXO, as a search model Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-
synapse
-
Homo sapiens 45202
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q12959
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged GK domain fragment of human DLG1 from Escherichia coli by anion exchange chromatography and gel filtration. The tag is cleaved by TEV protease Homo sapiens

General Information

General Information Comment Organism
evolution DLG1 is a member of the membrane associated guanylate kinase (MAGUK) family of proteins. The GK domains of the MAGUK family proteins are catalytically inactive, and instead are involved in protein-protein interactions Homo sapiens
additional information the DLG1 GK structure adopts an open conformation. Structural comparisons of the MAGUK guanylate kinase domains and the guanylate kinase enzyme, overview Homo sapiens
physiological function tumor suppressor discs large homolog 1, i.e. DLG1/SAP97, is involved in the development and regulation of neuronal and immunological synapses. DLG1 is a member of the membrane associated guanylate kinase (MAGUK) family of proteins, which function as molecular scaffolds. The C-terminal guanylate kinase (GK) domain of DLG1 binds peptides with a phosphorylated serine residue. The GK domains of the MAGUK family proteins are catalytically inactive, and instead are involved in protein-protein interactions Homo sapiens