Protein Variants | Comment | Organism |
---|---|---|
D54G | mutation identified in acute myocardial infarction patient, mutant shows substantially decreased activity, substrate binding affinity and stability | Homo sapiens |
D54G | the mutant enzyme has substantially decreased activity, substrate binding affinity and stability. Spectroscopic experiments indicate that the mutation impairs the structure of creatine kinase, which results in a partially unfolded state with more hydrophobic exposure and exposed Trp residues. The inability to fold to the functional compact state makes the mutant be prone to aggregate upon microenvironmental stresses, and might gradually decrease the creatine kinase level of the acute myocardial infarction patient | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
ATP | wild-type enzyme | Homo sapiens | |
0.46 | - |
ATP | wild-type | Homo sapiens | |
2.2 | - |
ATP | mutant D54G | Homo sapiens | |
2.2 | - |
ATP | mutant enzyme D54G | Homo sapiens | |
9.6 | - |
Creatine | wild-type enzyme | Homo sapiens | |
9.6 | - |
Creatine | wild-type | Homo sapiens | |
34 | - |
Creatine | mutant D54G | Homo sapiens | |
34 | - |
Creatine | mutant enzyme D54G | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
acute myocardial infarction patient | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52 | - |
mutant D54G | Homo sapiens |
52 | - |
mutant enzyme D54G | Homo sapiens |
245.5 | - |
wild-type enzyme | Homo sapiens |
246 | - |
wild-type | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | - |
Homo sapiens | ADP + phosphocreatine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
mutant D54G, melting temperature | Homo sapiens |
37 | - |
midpoint temperature of thermal inactivation of mutant enzyme D54G is 36.5°C | Homo sapiens |
45 | - |
10 min, mutant enzyme D54G is completely inactivated | Homo sapiens |
48 | - |
wild-type, stable up to 48°C | Homo sapiens |
48 | - |
10 min, the activity of wild-type enzyme has no significant changes after heat treatment at temperatures below 48°C | Homo sapiens |
52 | - |
wild-type, melting temperature | Homo sapiens |
53 | - |
midpoint temperature of thermal inactivation of wild-type enzyme is 52.5°C | Homo sapiens |
58 | - |
wild-type, complete loss of activity | Homo sapiens |
58 | - |
10 min, complete loss of activity above | Homo sapiens |