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Literature summary for 2.7.3.2 extracted from
- Over-expression, purification and characterization of the oligomerization dynamics of an invertebrate mitochondrial creatine kinase (2005), Biochim. Biophys. Acta, 1751, 184-193.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, optimization of overexpression of full length enzyme and mature enzyme lacking the mitochondrial targeting sequence in Escherichia coli | Chaetopterus variopedatus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Chaetopterus variopedatus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, recombinant enzyme, SDS-PAGE, dimer formation after dissociation of the octamer | Chaetopterus variopedatus |
| 43000 | - |
8 * 43000, recombinant enzyme, SDS-PAGE, enzyme primarily forms octamers | Chaetopterus variopedatus |
| 61500 | - |
dimeric enzyme form, gel filtration | Chaetopterus variopedatus |
| 347000 | - |
octameric enzyme form, gel filtration | Chaetopterus variopedatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | Chaetopterus variopedatus | the enzyme is involved in energy homeostasis | ADP + phosphocreatine | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chaetopterus variopedatus | - |
polychaete worm | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant full length enzyme and mature enzyme from Escherichia colito near homogeneity by affinity and cation exchange chromatography | Chaetopterus variopedatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Chaetopterus variopedatus | ADP + phosphocreatine | - |
r | |
| ATP + creatine | the enzyme is involved in energy homeostasis | Chaetopterus variopedatus | ADP + phosphocreatine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, recombinant enzyme, SDS-PAGE, dimer formation after dissociation of the octamer | Chaetopterus variopedatus |
| More | the octamer-dimer equilibrium highly depends on protein concentration and dilution, respectively, and on temperature, highest percentage of octamer occurs at 28°C, the lowest at 2°C, overview | Chaetopterus variopedatus |
| octamer | 8 * 43000, recombinant enzyme, SDS-PAGE, enzyme primarily forms octamers | Chaetopterus variopedatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MtCK | - |
Chaetopterus variopedatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Chaetopterus variopedatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Chaetopterus variopedatus | |
| ATP | - |
Chaetopterus variopedatus | |
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Release 2023.1 (January 2023)
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