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Literature summary for 2.7.3.2 extracted from
- Mitochondrial creatine kinase. Physical and kinetic properties of the purified enzyme from beef heart (1979), Biochemistry, 18, 1745-1751.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
ADP | 30°C | Bos taurus |  |
| 0.015 | - |
ADP | ADP in form of MgADP- | Bos taurus | |
| 0.056 | - |
ATP | 30°C | Bos taurus | |
| 0.056 | - |
ATP | ATP in form of MgATP2- | Bos taurus | |
| 0.31 | - |
creatine phosphate | 30°C | Bos taurus | |
| 4.5 | - |
Creatine | 30°C | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required as MgATP | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Bos taurus |
| 64000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20 mM sodium phosphate buffer, pH 8.0, 10% loss of activity in 2 weeks, faster inactivation in presence of dithiothreitol | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphocreatine | - |
Bos taurus | ATP + creatine | - |
r | |
| ATP + creatine | ATP required as MgATP2- | Bos taurus | ADP + creatine phosphate | - |
r | |
| ATP + creatine | - |
Bos taurus | ADP + phosphocreatine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | synthesis of MgATP2- | Bos taurus |
| 8 | - |
synthesis of phosphocreatine | Bos taurus |
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Release 2023.1 (January 2023)
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