Application | Comment | Organism |
---|---|---|
agriculture | enzyme does not exist in animals, good target for conception of new pesticides controlling weeds, fungi and bacteria | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
cDNA located on chromosome 4, cloned in Escherichia coli DH5alpha, overproduced in Escherichia coli BL21pLysS | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
ATP | pH 8.0, 30°C | Arabidopsis thaliana | |
11.6 | - |
L-aspartate | pH 8.0, 30°C | Arabidopsis thaliana | |
12.9 | - |
ATP | pH 8.0, 30°C, 0.5 M effector threonine | Arabidopsis thaliana | |
26.4 | - |
L-aspartate | pH 8.0, 30°C, 0.5 M effector threonine | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Arabidopsis thaliana | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
? * 93000, SDS-PAGE | Arabidopsis thaliana |
320000 | - |
gel filtration, in presence of 5.0 mM L-threonine | Arabidopsis thaliana |
470000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Arabidopsis thaliana | first and third step of the synthesis of methionine, catalyzed by a bifunctional enzyme | ADP + 4-phospho-L-aspartate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | O81852 | - |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.4 | - |
forward reaction | Arabidopsis thaliana |
18.87 | - |
reverse reaction | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-80°C, stored frozen without noticeable loss of enzyme activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Arabidopsis thaliana | ADP + 4-phospho-L-aspartate | - |
r | |
ATP + L-aspartate | first and third step of the synthesis of methionine, catalyzed by a bifunctional enzyme | Arabidopsis thaliana | ADP + 4-phospho-L-aspartate | - |
r |
Subunits | Comment | Organism |
---|---|---|
oligomer | ? * 93000, SDS-PAGE | Arabidopsis thaliana |
tetramer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AK-HSDH | - |
Arabidopsis thaliana |
aspartokinase-homoserine dehydrogenase | - |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | theoretical pI | - |
6.13 |