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Literature summary for 2.7.2.3 extracted from

  • Jones, C.E.; Fleming, T.M.; Cowan, D.A.; Littlechild, J.A.; Piper, P.W.
    The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli (1995), Eur. J. Biochem., 233, 800-808.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from Sulfolobus solfataricus overlap by 8-bp Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
x * 41000, SDS-PAGE Saccharolobus solfataricus
44395
-
x * 44395, calculated from sequence Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P50317
-
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment at 80°C is an effective first step in the purification of these recombinant enzyme from extracts of the Escherichia coli host Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.69
-
pH 7.5, 50°C Saccharolobus solfataricus

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Saccharolobus solfataricus
? x * 44395, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
PGK
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half life: 39 min Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharolobus solfataricus