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Literature summary for 2.7.2.3 extracted from
- Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells (2002), Indian J. Biochem. Biophys., 39, 332-341.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect by glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-phosphate, pyruvate, phosphoenolpyruvate and lactate | Mus musculus |
General Stability
| General Stability | Organism |
|---|---|
| optimal stabilization at 1 mM EDTA and 5 mM 2-mercaptoethanol | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibitory in forward reaction | Mus musculus |  |
| citrate | 40% inhibition at 50 mM | Mus musculus | |
| additional information | no effect by glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-phosphate, pyruvate, phosphoenolpyruvate and lactate | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values at different pH-values | Mus musculus | |
| 0.11 | - |
ATP | pH 6.9, 25°C | Mus musculus | |
| 0.2 | - |
3-phospho-D-glycerate | pH 6.9, 25°C | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cation | Mg2+ or Mn2+ | Mus musculus | |
| Mg2+ | 0.8 m Mg2+ and 0.5 mM Mn2+ are optimal | Mus musculus | |
| Mn2+ | 0.8 mM Mg2+ and 0.5 mM Mn2+ are optimal | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
Swiss albino mice | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 876fold, to homogeneity | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Ehrlich ascites carcinoma cell | from intraperitoneal cavity | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 169 | - |
purified enzyme | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Mus musculus | ADP + 1,3-diphosphoglycerate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 8 | 30% activity at pH 5.4, 60% activity at pH 8.0 | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Mus musculus | |
| ATP | required as phosphate donor | Mus musculus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
ADP | pH 6.5, 25°C, versus ATP | Mus musculus | |
| 0.175 | - |
ADP | pH 6.5, 25°C, versus 3-phospho-D-glycerate | Mus musculus | |
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