Literature summary for 2.7.2.3 extracted from

Zomer, A.W.; Allert, S.; Chevalier, N.; Callens, M.; Opperdoes, F.R.; Michels, P.A.
Purification and characterisation of the phosphoglycerate kinase isoenzymes of Trypanosoma brucei expressed in Escherichia coli (1998), Biochim. Biophys. Acta, 1386, 179-188.

Search for more literature for 2.7.2.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the glycosomal enzyme lacking the C-terminal glycosome-specific 20-residue signal sequence, in Escherichia coli	Trypanosoma brucei
overexpression of glycosomal and cytosolic isozymes in Escherichia coli BL21	Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
Ib1	isozymes are differntly sensitive	Trypanosoma brucei
suramin	isozymes are differently sensitive	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	ATP	recombinant cytosolic isozyme, pH 7.6, 25°C	Trypanosoma brucei
0.28	-	ATP	native glycosomal isozyme, pH 7.6, 25°C	Trypanosoma brucei
0.29	-	ATP	recombinant glycosomal isozyme, pH 7.6, 25°C	Trypanosoma brucei
0.33	-	ATP	recombinant truncated glycosomal isozyme, pH 7.6, 25°C	Trypanosoma brucei
0.46	-	ATP	native glycosomal isozyme, pH 7.6, 25°C	Trypanosoma brucei
1.25	-	3-phospho-D-glycerate	native cytosolic isozyme, pH 7.6, 25°C	Trypanosoma brucei
1.55	-	3-phospho-D-glycerate	native glycosomal isozyme, pH 7.6, 25°C	Trypanosoma brucei
2	-	3-phospho-D-glycerate	recombinant glycosomal isozyme and native cytosoli isozyme, pH 7.6, 25°C	Trypanosoma brucei
11.9	-	3-phospho-D-glycerate	recombinant truncated glycosomal isozyme, pH 7.6, 25°C	Trypanosoma brucei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	cytosolic isozyme	Trypanosoma brucei	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	x * 45000, cytosolic and truncated glycosomal isozymes, SDS-PAGE	Trypanosoma brucei
47000	-	x * 47000, glycosomal isozyme, SDS-PAGE	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	glycosomal PGKA and cytosolic PGKB isozymes	-

Purification (Commentary)

Purification (Comment)	Organism
2 isozymes and the truncated glycosomal isozyme, recombinant from Escherichia coli, to near homogeneity	Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
159	-	purified recombinant truncated glycosomal isozyme	Trypanosoma brucei
159	-	purified recombinant cytosolic isozyme	Trypanosoma brucei
1311	-	purified recombinant glycosomal isozyme	Trypanosoma brucei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 3-phospho-D-glycerate	-	Trypanosoma brucei	ADP + 1,3-diphosphoglycerate	-	r

Subunits

Subunits	Comment	Organism
?	x * 47000, glycosomal isozyme, SDS-PAGE	Trypanosoma brucei
dimer	x * 45000, cytosolic and truncated glycosomal isozymes, SDS-PAGE	Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Trypanosoma brucei

Cofactor

Cofactor	Comment	Organism	Structure
ADP	-	Trypanosoma brucei
ATP	required as phosphate donor	Trypanosoma brucei

pI Value

Organism	Comment	pI Value Maximum	pI Value
Trypanosoma brucei	native cytosolic isozyme, isoelectric focusing	6.8	6.3
Trypanosoma brucei	recombinant cytosolic isozyme, isoelectric focussing	-	6.5
Trypanosoma brucei	truncated glycosomal isozyme, isoelectric focusing	-	9.2
Trypanosoma brucei	glycosomal isozyme, isoelectric focusing	-	9.3

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Release 2023.1 (January 2023)