Cloned (Comment) | Organism |
---|---|
expression of the glycosomal enzyme lacking the C-terminal glycosome-specific 20-residue signal sequence, in Escherichia coli | Trypanosoma brucei |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion method, protein solution containing 2.5 M potassium sodium phosphate, pH 8.0, X-ray diffraction structure determination and analysis | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 3-phospho-D-glyceroyl phosphate | Trypanosoma brucei | key enzyme in glycolysis | ATP + 3-phospho-D-glycerate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli, to homogeneity | Trypanosoma brucei |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate | mechanism, substrate-induced effects combine synergistically to induce major conformational changes of the active site to catalytic status | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 3-phospho-D-glyceroyl phosphate | key enzyme in glycolysis | Trypanosoma brucei | ATP + 3-phospho-D-glycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Trypanosoma brucei | ADP + 1,3-diphosphoglycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | ternary structure | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Trypanosoma brucei | |
ATP | required as phosphate donor | Trypanosoma brucei |