KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | biphasic kinetics | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
cytosol | - |
Spinacia oleracea | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
gel filtration, cytosolic and chloroplastic isoenzyme | Spinacia oleracea |
40700 | - |
1 * 40700, cytosolic isozyme, SDS-PAGE | Spinacia oleracea |
41000 | - |
1 * 41000, chloroplastic isozyme, SDS-PAGE | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
2 isozymes, cytosolic and chloroplastic | - |
Spinacia oleracea | - |
Sorte Noveto | - |
Purification (Comment) | Organism |
---|---|
cytosolic, 160fold, and chloroplastic, 200fold, isozymes, to homogeneity | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
798 | - |
purified cytosolic isozyme | Spinacia oleracea |
800 | - |
purified chloroplastic isozyme | Spinacia oleracea |
Storage Stability | Organism |
---|---|
4°C, enzyme in the fractions from the phenyl-Sepharose column, stable for at least 2 months | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Spinacia oleracea | ADP + 1,3-diphosphoglycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40700, cytosolic isozyme, SDS-PAGE | Spinacia oleracea |
monomer | 1 * 41000, chloroplastic isozyme, SDS-PAGE | Spinacia oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Spinacia oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
unstable above | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
both isozymes | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Spinacia oleracea | |
ATP | required as phosphate donor | Spinacia oleracea |