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Literature summary for 2.7.2.3 extracted from
- Phosphoglycerate kinase from Bacillus stearothermophilus (1982), Methods Enzymol., 90, 126-130.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 58% | Geobacillus stearothermophilus |  |
| nucleoside diphosphates | purine nucleotide diphosphates | Geobacillus stearothermophilus | |
| nucleoside monophosphates | purine nucleotide monophosphates | Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Geobacillus stearothermophilus | |
| 2.9 | - |
ATP | pH 7.6, 25°C | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
| Ca2+ | 15% of the activity with Mg2+ | Geobacillus stearothermophilus | |
| Co2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
| Co2+ | 58% of the activity with Mg2+ | Geobacillus stearothermophilus | |
| divalent cation | Mg2+ or Mn2+ | Geobacillus stearothermophilus | |
| Mg2+ | - |
Geobacillus stearothermophilus | |
| Mn2+ | Mn2+ or Mg2+ required | Geobacillus stearothermophilus | |
| additional information | no activation by Ba2+ | Geobacillus stearothermophilus | |
| Ni2+ | no activation | Geobacillus stearothermophilus | |
| Zn2+ | no activation | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
NCA 1503 | - |
| Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 557 | - |
purified enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | absolute specificity for 3-phospho-D-glycerate | Geobacillus stearothermophilus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | absolute specificity for 3-phospho-D-glycerate | Geobacillus stearothermophilus NCA 1503 | ADP + 1,3-diphosphoglycerate | - |
r | |
| GTP + 3-phospho-D-glycerate | 27% of the activity with ATP | Geobacillus stearothermophilus | GDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | 27% of the activity with ATP | Geobacillus stearothermophilus NCA 1503 | GDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | 42% of the activity with ATP | Geobacillus stearothermophilus | IDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | 42% of the activity with ATP | Geobacillus stearothermophilus NCA 1503 | IDP + 1,3-diphosphoglycerate | - |
? | |
| additional information | no activity with pyrimidine nucleotides | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | no activity with pyrimidine nucleotides | Geobacillus stearothermophilus NCA 1503 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Geobacillus stearothermophilus |
| More | secondary structure: 20% alpha-helix, 45% beta-structure | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Geobacillus stearothermophilus |
| 55 | 60 | physiological temperature | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, stable | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
inactivation below | Geobacillus stearothermophilus |
| 6 | - |
stable above | Geobacillus stearothermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Geobacillus stearothermophilus | |
| ATP | true substrate is the magnesium complexes of ATP | Geobacillus stearothermophilus | |
| ATP | required as phosphate donor | Geobacillus stearothermophilus | |
| GTP | - |
Geobacillus stearothermophilus | |
| ITP | - |
Geobacillus stearothermophilus | |
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