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Literature summary for 2.7.2.3 extracted from
- Phosphoglycerate kinase from the extreme thermophile Thermus thermophilus. Crystallization and preliminary X-ray data (1987), FEBS Lett., 225, 123-126.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| partial DNA and amino acid sequence determination | Thermus thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization from ammonium sulfate precipitation with 37% w/v by hanging or sitting drop vapour diffusion method, protein solution: 4 mg/ml, PIPES buffer, pH 6.8, 10 mM MgCl2, 1 mM ATP, 10% (NH4)2SO4, 22°C, 7-14 days, high resolution X-ray diffraction structure determination and analysis, rotating camera system | Thermus thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000 | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + 3-phospho-D-glyceroyl phosphate | Thermus thermophilus | - |
ATP + 3-phospho-D-glycerate | - |
r |  |
| ADP + 3-phospho-D-glyceroyl phosphate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
ATP + 3-phospho-D-glycerate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + 3-phospho-D-glyceroyl phosphate | - |
Thermus thermophilus | ATP + 3-phospho-D-glycerate | - |
r | |
| ADP + 3-phospho-D-glyceroyl phosphate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ATP + 3-phospho-D-glycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Thermus thermophilus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + 1,3-diphosphoglycerate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000 | Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
stable | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Thermus thermophilus | |
| ATP | required as phosphate donor | Thermus thermophilus | |
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