General Stability | Organism |
---|---|
extensive dialysis against 0.1 M Tris/HCl, pH 7.6, 1 mM DTT, 1 mM EDTA, 1 mM sodium azide, 0.2 M ammonium sulfate, stable | Trypanosoma brucei |
freezing inactivates | Trypanosoma brucei |
stable as a suspension in ammonium sulfate, 70-90% saturation | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma brucei | 20015 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
bloodstream form, stock 427 | - |
Purification (Comment) | Organism |
---|---|
660fold, to homogeneity | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
504 | - |
purified enzyme | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Trypanosoma brucei | ADP + 1,3-diphosphoglycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Trypanosoma brucei | |
ATP | required as phosphate donor | Trypanosoma brucei |