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Literature summary for 2.7.2.11 extracted from

  • Morita, Y.; Nakamori, S.; Takagi, H.
    L-proline accumulation and freeze tolerance of Saccharomyces cerevisiae are caused by a mutation in the PRO1 gene encoding gamma-glutamyl kinase (2003), Appl. Environ. Microbiol., 69, 212-219.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology the D154N mutation results in a prominant increase in cell viability after freezing at -20°C compared to the viability of the cells harboring the wild-type PRO1 gene, method for breeding novel freeze-tolerant yeast strains Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D154N the mutation results in a prominant increase in cell viability after freezing at -20°C compared to the viability of the cells harboring the wild-type PRO1 gene. The altered gamma-glutamyl kinase results in stabilization of the complex with gamma-glutamyl phosphate reductase or has an indirect effect on gamma-glutamyl phosphate reductase activity which leads to an increase in L-proline production in Saccharomyces cerevisiae Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32264
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate
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Saccharomyces cerevisiae ADP + L-glutamate 5-phosphate
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