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Literature summary for 2.7.2.11 extracted from

  • Smith, C.J.; Deutch, A.H.; Rushlow, K.E.
    Purification and characteristics of a gamma-glutamyl kinase involved in Escherichia coli proline biosynthesis (1984), J. Bacteriol., 157, 545-551.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.11

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 ATP pH 7.0, 37°C, gamma-glutamyl kinase DHPr Escherichia coli
0.5
-
 ATP pH 7.0, 37°C, gamma-glutamyl kinase w+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 6 * 40000, gamma-glutamyl kinase DHPr, SDS-PAGE Escherichia coli
236000
-
 gamma-glutamyl kinase DHPr, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate Escherichia coli the enzyme catalyzes the first step in the pathway from glutamate to proline ADP + L-glutamate 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from strain BRL806, designated as gamma-glutamyl kinase w+ and from reductase-overproducing strain BRL1945, designated as gamma-glutamyl kinase DHPr Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate
-
 Escherichia coli ADP + L-glutamate 5-phosphate
-
 ?
ATP + L-glutamate the enzyme catalyzes the first step in the pathway from glutamate to proline Escherichia coli ADP + L-glutamate 5-phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 40000, gamma-glutamyl kinase DHPr, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 50% of maximal activity at pH 6.0 and at pH 7.5 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
 ADP pH 7.0, 37°C Escherichia coli