Any feedback?
Literature summary for 2.7.12.2 extracted from
- Manipulation of mitogen-activated protein kinase kinase signaling in the Arabidopsis stomatal lineage reveals motifs that contribute to protein localization and signaling specificity (2014), Plant Cell, 26, 3358-3371.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MKK4, recombinant expression using a cell type-specific expression system is unique in that it allows activation of MKK signaling in cells exhibiting important generalizable behaviors (e.g., cell division and fate acquisition) without the induction of cell death or additional deleterious phenotypes commonly associated with broad activation of MKKs | Arabidopsis thaliana |
| gene MKK5, recombinant expression using a cell type-specific expression system is unique in that it allows activation of MKK signaling in cells exhibiting important generalizable behaviors (e.g., cell division and fate acquisition) without the induction of cell death or additional deleterious phenotypes commonly associated with broad activation of MKKs | Arabidopsis thaliana |
| gene MKK7, recombinant expression using a cell type-specific expression system is unique in that it allows activation of MKK signaling in cells exhibiting important generalizable behaviors (e.g., cell division and fate acquisition) without the induction of cell death or additional deleterious phenotypes commonly associated with broad activation of MKKs | Arabidopsis thaliana |
| gene MKK9, recombinant expression using a cell type-specific expression system is unique in that it allows activation of MKK signaling in cells exhibiting important generalizable behaviors (e.g., cell division and fate acquisition) without the induction of cell death or additional deleterious phenotypes commonly associated with broad activation of MKKs | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a MKK4 deletion variant | Arabidopsis thaliana |
| additional information | construction of a MKK5 deletion variant | Arabidopsis thaliana |
| additional information | construction of a MKK7 deletion variant | Arabidopsis thaliana |
| additional information | construction of a MKK9 deletion variant | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
| mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | Arabidopsis thaliana | - |
ADP + a phosphoprotein | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | stomata | Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | - |
Arabidopsis thaliana | ADP + a phosphoprotein | - |
? | |
| ATP + KIMPK3 | - |
Arabidopsis thaliana | ADP + phosphorylated KIMPK3 | - |
? | |
| ATP + KIMPK6 | - |
Arabidopsis thaliana | ADP + phosphorylated KIMPK6 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the D-domain of MKK4 is preceded by a conserved 14-amino acid extension and followed by a 10-amino acid insertion before the about 30-amino acid stretch | Arabidopsis thaliana |
| More | the D-domain of MKK5 is preceded by a conserved 14-amino acid extension and followed by a 10-amino acid insertion before the about 30-amino acid stretch | Arabidopsis thaliana |
| More | the D-domain of MKK7 is located near the N terminus of the protein and is separated from the canonical kinase domain by about 30 amino acids | Arabidopsis thaliana |
| More | the D-domain of MKK9 is located near the N terminus of the protein and is separated from the canonical kinase domain by about 30 amino acids | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MKK4 | - |
Arabidopsis thaliana |
| MKK5 | - |
Arabidopsis thaliana |
| MKK7 | - |
Arabidopsis thaliana |
| MKK9 | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | D-domain mutant CAMKK7DELTAD is inactive toward MPK6 but the activity toward MPK3 is maintained, and the CAMKK7DELTAD mutant loses stomatal cluster-promoting activity | Arabidopsis thaliana |
| malfunction | in both CAMKK4 and CAMKK5, removal of the entire N terminus (CAMKK4/5DELTANextDNint) renders the kinases incapable of inhibiting development during the SPCH and MUTE stages. As with CAMKK4/5 variants lacking D-domains, CAMKK7/9DD variants are generally unable to inhibit stomatal development in the SPCH and MUTE stages | Arabidopsis thaliana |
| additional information | installation of a cell type-specific expression system, unique in that it allows activation of MKK signaling in cells exhibiting important generalizable behaviors (e.g., cell division and fate acquisition) without the induction of cell death or additional deleterious phenotypes commonly associated with broad activation of MKKs. Determination of influence of functional docking domains of MKKs on MAPK signal output, overview | Arabidopsis thaliana |
| additional information | installation of a cell type-specific expression system, unique in that it allows activation of MKK signaling in cells exhibiting important generalizable behaviors (e.g., cell division and fate acquisition) without the induction of cell death or additional deleterious phenotypes commonly associated with broad activation of MKKs. Determination of influence of functional docking domains of MKKs on MAPK signal output, overview. The D-domains of MKK7 regulate the subcellular localization of the kinase, overview | Arabidopsis thaliana |
| additional information | installation of a cell type-specific expression system, unique in that it allows activation of MKK signaling in cells exhibiting important generalizable behaviors (e.g., cell division and fate acquisition) without the induction of cell death or additional deleterious phenotypes commonly associated with broad activation of MKKs. Determination of influence of functional docking domains of MKKs on MAPK signal output, overview. The D-domains of MKK9 regulate the subcellular localization of the kinase, overview | Arabidopsis thaliana |
| physiological function | isozymes MKK4/5/7/9 affect stomatal development, the D-domains of MKK7/9 are important in regulating subcellular localization of the kinases and this localization is necessary for stomatal promoting activity, overview. Additional regions of MKK4/5 function as MKK specificity determinants in vivo. A 14-3-3 binding site in the D-domain of MKK4 contributes to MKK4 function. When rendered constitutively active, four MAPK kinases (MKKs), MKK4/5/7/9, are capable of perturbing stomatal development, the kinases comprise two pairs, MKK4/5 and MKK7/9, with both overlapping and divergent functions. The behavior of MKK4 mirrors that of MKK5 and MKK7 mirrors that of MKK9 in the stomatal lineage | Arabidopsis thaliana |
| physiological function | isozymes MKK4/5/7/9 affect stomatal development, the D-domains of MKK7/9 are important in regulating subcellular localization of the kinases and this localization is necessary for stomatal promoting activity, overview. Additional regions of MKK4/5 function as MKK specificity determinants in vivo. When rendered constitutively active, four MAPK kinases (MKKs), MKK4/5/7/9, are capable of perturbing stomatal development, the kinases comprise two pairs, MKK4/5 and MKK7/9, with both overlapping and divergent functions. The behavior of MKK4 mirrors that of MKK5 and MKK7 mirrors that of MKK9 in the stomatal lineage | Arabidopsis thaliana |
| physiological function | isozymes MKK4/5/7/9 affect stomatal development, the D-domains of MKK7/9 are important in regulating subcellular localization of the kinases and this localization is necessary for stomatal promoting activity, overview. When rendered constitutively active, four MAPK kinases (MKKs), MKK4/5/7/9, are capable of perturbing stomatal development, the kinases comprise two pairs, MKK4/5 and MKK7/9, with both overlapping and divergent functions. The behavior of MKK4 mirrors that of MKK5 and MKK7 mirrors that of MKK9 in the stomatal lineage | Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
