Cloned (Comment) | Organism |
---|---|
recombinant expression of a fusion protein between SIP2 and the N-terminal CFP (SIP2:SCFPN173) in Nicotiana benthamiana epidermis leaves via Agrobacterium tumefaciensmediated transient transformation. Recombinant expression of polyHis- or GST-tagged wild-type and mutant enzymes in Escherichia coli. Real-time PCR expression analysis | Lotus japonicus |
Protein Variants | Comment | Organism |
---|---|---|
K120R | generation of kinase-negative mutants of both protein kinases SymRK and SIP2 by replacing the essential Lys residue with Arg at the ATP binding site. This Lys-to-Arg substitution (SymRK-PK-KR and SIP2-KR) abolishes the kinase activity completely, but does not affect the interaction between SymRK and SIP2 | Lotus japonicus |
additional information | construction of SIP2 RNA interference (RNAi) knockdown plants. Construction of plasmids that express a fusion protein between SIP2 and the N-terminal CFP (SIP2:SCFPN173). The constructs are co-expressed in Nicotiana benthamiana epidermis leaves via Agrobacterium tumefaciens-mediated transient transformation | Lotus japonicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | SymRK acts as an inhibitor of SIP2 kinase activity when MPK6 is used as a substrate, suggesting that SymRK may serve as a negative regulator of the SIP2 signaling pathway. SymRK and SIP2 interact with each other but cannot use one another as a substrate for phosphorylation. SymRK can autophosphorylate itself but fails to phosphorylate the kinase-negative SIP2-KR, suggesting that SymRK is neither a potential phosphorylation target nor a kinase source of SIP2 | Lotus japonicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Lotus japonicus | 5737 | - |
plasma membrane | - |
Lotus japonicus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Lotus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lotus japonicus | F8THZ4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Lotus japonicus | - |
additional information | gene SIP2 is constitutively expressed in all Lotus japonicus tissues examined | Lotus japonicus | - |
root | - |
Lotus japonicus | - |
root nodule | - |
Lotus japonicus | - |
stem | - |
Lotus japonicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | - |
Lotus japonicus | ADP + phosphorylated casein | - |
? | |
ATP + MAP kinase 6 | Arabidopsis thaliana MAP kinase MPK6 | Lotus japonicus | ADP + phosphorylated Map kinase 6 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAP kinase kinase | - |
Lotus japonicus |
MAPKK | - |
Lotus japonicus |
Sip2 | - |
Lotus japonicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Lotus japonicus |
Organism | Comment | Expression |
---|---|---|
Lotus japonicus | hairy roots expressing SIP2 RNAi have downregulated SIP2 mRNA levels in about 70% of transgenic hairy roots | down |
Lotus japonicus | inoculation with Mesorhizobium loti MAFF303099 does not induce the SIP2 enzyme expression | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown expression of SIP2 via RNA interference (RNAi) results in drastic reduction of nodules formed in transgenic hairy roots. In these roots, the expression levels of SIP2 and three marker genes for infection thread and nodule primordium formation are downregulated drastically, while the expression of two other MAPKK genes are not altered | Lotus japonicus |
additional information | the interaction between SymRK and SIP2 is conserved among legumes, interaction analysis of SIP2 and SymRK from different legume species, overview | Lotus japonicus |
physiological function | SIP2 represents a typical plant mitogen-activated protein kinase kinase (MAPKK) and exhibits autophosphorylation and transphosphorylation activities. SymRK may serve as a negative regulator of the SIP2 signaling pathway. SymRK and SIP2 interaqct and are both protein kinases. The kinase domain of SIP2 is responsible for its interaction with SymRK. Essential role of SIP2 in the early symbiosis signaling and nodule organogenesis | Lotus japonicus |