Literature summary for 2.7.12.2 extracted from

Wang, C.; Lu, W.; He, X.; Wang, F.; Zhou, Y.; Guo, X.; Guo, X.
The cotton mitogen-activated protein kinase kinase 3 functions in drought tolerance by regulating stomatal responses and root growth (2016), Plant Cell Physiol., 57, 1629-1642.

Cloned(Commentary)

Cloned (Comment)	Organism
cloning of cotton group B MAPKK gene GhMKK3, the gene has 6 introns, sequence comparisons and phylogenetic analysis, overexpression in Nicotiana benthamiana, RNA sequencing and quantitative real-time PCR analysis. fusion Transient expression of the GFP-tagged enzyme in Nicotiana benthamiana leaves using Agrobacterium infiltration (agroinfiltration) or transiently transformed into onion epidermal cells using particle bombardment	Gossypium hirsutum

Cloned (Comment)

Organism

cloning of cotton group B MAPKK gene GhMKK3, the gene has 6 introns, sequence comparisons and phylogenetic analysis, overexpression in Nicotiana benthamiana, RNA sequencing and quantitative real-time PCR analysis. fusion Transient expression of the GFP-tagged enzyme in Nicotiana benthamiana leaves using Agrobacterium infiltration (agroinfiltration) or transiently transformed into onion epidermal cells using particle bombardment

Gossypium hirsutum

Protein Variants

Protein Variants	Comment	Organism
additional information	overexpression of GhMKK3 enhances plant drought tolerance. Overexpression of GhMKK3 promotes abscisic acid-induced stomatal closure and decreases stomatal density. Phenotype, overview	Gossypium hirsutum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Gossypium hirsutum	5737	-
nucleus	-	Gossypium hirsutum	5634	-
protoplast	-	Gossypium hirsutum	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Gossypium hirsutum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57523	-	-	Gossypium hirsutum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + GhMPK7	Gossypium hirsutum	-	ADP + phosphorylated GhMPK7	-	?
additional information	Gossypium hirsutum	GhMKK3 interacts with GhMPK7, a typical C group MAPK member, and GhPIP1 potein. GhMPK7 is phosphorylated by GhMKK3 in protoplasts	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Gossypium hirsutum	S4S5K3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + GhMPK7	-	Gossypium hirsutum	ADP + phosphorylated GhMPK7	-	?
additional information	GhMKK3 interacts with GhMPK7, a typical C group MAPK member, and GhPIP1 potein. GhMPK7 is phosphorylated by GhMKK3 in protoplasts	Gossypium hirsutum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 57523, sequence calculation	Gossypium hirsutum

Synonyms

Synonyms	Comment	Organism
GhMKK3	-	Gossypium hirsutum
MAPKK3	-	Gossypium hirsutum
mitogen-activated protein kinase kinase 3	-	Gossypium hirsutum

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Gossypium hirsutum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Gossypium hirsutum	sequence calculation	-	5.84

Expression

Organism	Comment	Expression
Gossypium hirsutum	osmotic stress and NaCl cause a significant decrease in GhMKK3 transcript levels. GhMKK3 expression is downregulated by treatment with hydrogen peroxide, abscisic acid, and with mannitol	down
Gossypium hirsutum	gibberellic acid induces the enzyme expression	up

General Information

General Information	Comment	Organism
metabolism	mitogen-activated protein kinase (MAPK) cascades play critical roles in signal transduction processes in eukaryotes. The MAPK kinases (MAPKKs) that link MAPKK kinases (MAPKKKs) and MAPKs are key components of MAPK cascades	Gossypium hirsutum
physiological function	GhMKK3 plays an important role in drought tolerance in cotton. Expression of GhPIP1 and GhMPK7 is induced in a MKK3-dependent manner by abscisic acid and drought	Gossypium hirsutum