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Literature summary for 2.7.12.2 extracted from

  • Jo, C.; Cho, S.J.; Jo, S.A.
    Mitogen-activated protein kinase kinase 1 (MEK1) stabilizes MyoD through direct phosphorylation at tyrosine 156 during myogenic differentiation (2011), J. Biol. Chem., 286, 18903-18913.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.12.2

Cloned(Commentary)

Cloned (Comment) Organism
recombinant coexpression of His6-tagged constitutively active MEK1 mutant S218E/S222E and GST-MyoD protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
S218E/S222E site-directed mutagenesis, a constitutively active MEK1 mutant, i.e. MEK1EE Homo sapiens
Y156F site-directed mutagenesis, catalytically inactive mutant. The protein level of mutant MyoD-Y156F decreases compared with that of wild type but is recovered in the presence of lactacystin, a proteasome inhibitor. The low protein level of MyoD-Y156F is recovered over that of wild-type by an additional mutation at Leu164, a critical binding residue of MAFbx/AT-1, a Skp, Cullin, F-box (SCF) E3-ubiquitin ligase Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
PD184352 a MEK-specific inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus activated MEK1 associates with MyoD in the nucleus of differentiating myoblasts Homo sapiens 5634
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + MyoD Homo sapiens phosphorylation at Tyr156, activated MEK1 associates with MyoD ADP + phospharylated MyoD
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q02750
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Source Tissue

Source Tissue Comment Organism Textmining
C2C12 cell
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 Homo sapiens
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myoblast activated MEK1 associates with MyoD in the nucleus of differentiating myoblasts Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + MyoD phosphorylation at Tyr156, activated MEK1 associates with MyoD Homo sapiens ADP + phospharylated MyoD
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 ?
ATP + MyoD phosphorylation at Tyr156 recombinant GST-tagged MyoD substrate. Mutational analysis of phosphorylation sites using MyoD Tyr mutants, overview Homo sapiens ADP + phospharylated MyoD
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 ?

Synonyms

Synonyms Comment Organism
MEK1
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 Homo sapiens
mitogen-activated protein kinase kinase 1
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 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function transcriptional activity of MyoD is critical in understanding skeletal muscle development and regeneration. Mitogen-activated protein kinase kinase 1 (MEK1) stabilizes MyoD through direct phosphorylation at tyrosine 156 during myogenic differentiation. The amount of MyoD co-precipitated with MAFbx/AT-1 is reduced in the presence of active MEK1. The phosphorylation probably interrupts the binding of MAFbx/AT-1 to MyoD and thereby increases its stability. The MEK1-induced phosphorylation of MyoD enhances heterodimerization with E proteins Homo sapiens